Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Organ-specific extracellular matrix directs trans-differentiation of mesenchymal stem cells and formation of salivary gland-like organoids in vivo

Fig. 1Fig. 1

Light and immunofluorescence microscopy and proteomic analysis showed that decellularization removed cell nuclei from SMG tissue and left ECM components intact. A Hematoxylin and eosin (H&E) staining of SMG tissue sections showed that decellularization removed the vast majority of cell nuclei (blue). Immunofluorescence (IF) staining for type IV collagen (red) showed that decellularization left the ECM intact, while DAPI staining (blue) showed that cell nuclei were removed. Staining with nonspecific isotype antibodies was used as a negative control (not shown). Bar: 50 µm. B Analysis of the protein components present in SMG tissue before and after decellularization using mass spectrometry. The Venn diagram shows the number of unique proteins identified in SMG before and after decellularization. The area of intersection shows the number of identical proteins retained in both samples. In the table, proteins identified by proteomic analysis have been classified based on their functionality (“SG tissue,” “ECM,” or “Other”) using the Jensen TISSUES database. The percent of proteins in each class is shown. Note: ECM data were further analyzed, using normalized total ion current (TIC), to obtain an estimate of the quantity of proteins present and a percent calculated; this is shown as “ECM-portion*.” Decellularization enriched the absolute quantity of ECM proteins present. C List of unique ECM proteins in SMG tissue before cell removal. The data are expressed as normalized TIC. D List of retained ECM proteins in SMG tissue after cell removal and a calculation of the relative fold change (log2 [TIC after cell removal/TIC before cell removal]). E List of unique ECM proteins in SMG tissue after cell removal. The data are expressed as normalized TIC

Back to article page