Fig. 1
Hypoxia favors hBMSC survival and HIF-1α-mediated TGF-β1 secretion. a Western blotting and b quantitative analysis were used to analyze the expression level of HIF-1α in hBMSCs after different times (12, 24 and 48 h) of hypoxia (1% O2) and normoxia. c Representative fluorescence images of HIF-1α-stained hBMSCs under different hypoxic and normoxic conditions. Bar, 50 μm. d The viability of hBMSCs was determined using MTS assays after hypoxia and normoxia for different times (12, 24, 36 and 48 h). e Western blotting and f quantitative analysis were used to analyze the expression levels of TGF-β1 in hBMSCs after 48 h of hypoxic and normoxic conditions. g ELISA was performed to measure the protein level of secreted TGF-β1 in CM from hBMSCs subjected to 48 h of hypoxic and normoxic conditions. h Western blotting and i quantitative analysis were used to analyze the expression levels of HIF-1α and TGF-β1 in control hBMSCs and siHIF-1α hBMSCs after 48 h of hypoxia. j ELISA was performed to measure TGF-β1 secretion levels in the CM of control hBMSCs and siHIF-1α hBMSCs after 48 h of hypoxia treatment. Mean ± SEM. n = 3. *P < 0.05, **P < 0.01