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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Bone marrow mesenchymal stem cells facilitate diabetic wound healing through the restoration of epidermal cell autophagy via the HIF-1α/TGF-β1/SMAD pathway

Fig. 3

The autophagic effect of hypoxic hBMSCs promotes HaCaT cell proliferation and migration. a qRT-PCR and b, c Western blotting was used to measure the mRNA and protein levels of ATG5 and ATG7 to evaluate the efficiency of siATG5 and siATG7 in HaCaT cells at 24 h after hyCM administration. d Western blotting and e quantitative analysis were used to analyze the expression levels of LC3B-I/II and SQSTM1 in control HaCaT cells and siATG5 and siATG7 HaCaT cells after the cells were treated with different conditioned media for 24 h. f MTS and g EdU assays were used to assess the proliferation of these HaCaT cells after 24 h of treatment with different CM. Bar, 100. h Scratch assays and i quantitative analysis were performed to detect the migration of HaCaT cells after treatment with different conditioned media for 24 h. Bar, 200 μm. Mean ± SEM. n = 3. *P < 0.05, **P < 0.01

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