Fig. 4

hBMSC-derived TGF-β1 contributes to the activation of HaCaT cell autophagy by hypoxic hBMSCs. a Western blotting and b quantitative analysis were used to measure the protein level of TGF-β1 to evaluate the efficiency of siTGF-β1 in hBMSCs after 24 h of hypoxia exposure. c ELISA was performed to measure the protein level of secreted TGF-β1 in the CM from control hBMSCs and siTGF-β1 hBMSCs after 24 h of hypoxia. d Western blotting and e quantitative analysis were used to analyze the expression levels of ATG5, ATG7, LC3B-I/II, BECN1 and SQSTM1 in HaCaT cells after 24 h of exposure to CM from control hy-hBMSCs and siTGF-β1 hy-hBMSCs. Mean ± SEM. n = 3. *P < 0.05, **P < 0.01