Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: A defined road to tracheal reconstruction: laser structuring and cell support for rapid clinic translation

Fig. 1

The study’s design. The samples of the donor trachea were isolated from the human cadaver material, washed, and removed from the adipose tissue. Then, they were decellularized, cross-linked with EGDE, and treated with laser to form wells on their surface. The formed matrices were seeded with human MSC and cultured in the growth medium up to 12 days. The fabricated cartilage constructs were implanted heterotopically into nude, immunosuppressed, and normal mice and orthotopically into rabbits. This study showed that the developed cartilage constructs were highly biocompatible and efficient in treating the tracheal defects. Therefore, this research can be considered as one of first steps in the clinical translation of this technology. Abbreviations: DC—decellularized; CL—cross-linked

Back to article page