Fig. 5

Notch-activated MSCs inhibit XBP1s/NLRP3 activation in an AMPK/SIRT1-dependent manner. JAG1-MSCs-treated mice were injected via the tail vein with SIRT1 siRNA (siSIRT1; 2 mg/kg) or non-specific control siRNAs (siNS) mixed with mannose-conjugated polymers 4 h prior to drug induction. A Western blot analysis of p-AMPKα, AMPKα and SIRT1 expression in liver tissues. B Western blot analysis of SIRT1, XBP1s, NLRP3, ASC and C-caspase-1 protein expression in liver tissues. C, D ELISA analysis of serum IL-1β and IL-18 levels. E Representative histological staining (H&E) of liver tissue (n = 5 mice/group) and percent of necrotic area. Scale bars: 100 μm. F Hepatocellular function, as assessed by serum ALT levels (IU/L). Data are presented as the mean ± SD. *p < 0.05, **p < 0.01