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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Notch-activated mesenchymal stromal/stem cells enhance the protective effect against acetaminophen-induced acute liver injury by activating AMPK/SIRT1 pathway

Fig. 6

Notch-activated MSCs promote XBP1s deacetylation and inhibit XBP1s/NLRP3 activation in macrophages. Bone marrow-derived macrophages (BMDMs, 1 × 106) were treated with MSC-derived conditioned media (MSC-CM) for 24 h followed by LPS (100 ng/ml) stimulation. A Western blot analysis of p-AMPKα, AMPKα, SIRT1, XBP1s, NLRP3 and C-caspase-1 expression in BMDMs. B ELISA analysis of IL-1β and IL-18 levels from BMDM supernatant. C Western blot analysis of p-AMPKα, AMPKα, SIRT1, XBP1s, NLRP3 and C-caspase-1 expression in BMDMs. D BMDMs were transfected with NS siRNA or SIRT1 siRNA and then treated with JAG1-MSC-CM. The XBP1s acetylation level was evaluated by performing immunoprecipitation (IP) with anti-acetylation antibody and Western blotting with anti-XBP1s antibody. The expressions of XBP1s, NLRP3 and C-caspase-1 in the whole-cell lysates were examined by Western blot analysis. All data are presented as means ± SD. *p < 0.05; **p < 0.01

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