Fig. 6
hUSC-sEVs activated the ERK1/2 and PI3K/AKT signaling pathways by delivering miR-126-3p. A Normalized miRNA expression levels of hUSC-sEVs measured by miRNA microarray. B The predicted miRNA expression levels of hUSC-sEVs measured by qPCR. C VK2 cells were treated with hUSC-sEVs for 6 h, and the expression of the predicted miRNAs was measured by qPCR. D The potential target genes of miR-126-3p were predicted by bioinformatics analysis. E VK2 cells were transfected with miR-126-3p for 36 h, and then the mRNA expression of Spred1 and PIK3R2 in VK2 cells was measured by qPCR. F VK2 cells were treated with hUSC-sEVs for 36 h, and the protein levels of Spred1, ERK1/2, p-ERK1/2, PIK3R2, AKT, and p-AKT in VK2 cells were analyzed by western blotting. The PBS and sEVs groups indicated 0 and 10 × 108 hUSC-sEVs particles/mL. *P < 0.05, **P < 0.01, ***P < 0.001