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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Extracellular vesicles derived from human umbilical cord mesenchymal stem cells alleviate osteoarthritis of the knee in mice model by interacting with METTL3 to reduce m6A of NLRP3 in macrophage

Fig. 3

The protection effect of knee OA by hucMSCs-EVs was abolished in NLRP3−/− mice. A. Safranin O and Fast Green staining of knee sections and micro-CT analysis of knee joint in NLRP3−/− mice with DMM-induced OA. Expression levels of COL2A1, Aggrecan, ADAMTS5, and MMP13 were determined by immunofluorescence staining. Scale bar = 400 μm for Safranin O and Fast Green staining; Scale bar = 1 mm for Micro-CT; Scale bar = 100 μm for immunofluorescence staining. B. OARIS scores of knee sections in each group (n = 10). C. Osteophyte score of each group (n = 10). D. Quantification of mean fluorescence intensity of COL2A1, Aggrecan, ADAMTS5, and MMP13 in each group (n = 10). E. Levels of IL-1β, IL-18, and TNF-α in mice knee tissue as determined by ELISA (n = 3). Data are presented as mean ± SD. ns, no significance; *p < 0.05; **p < 0.01; ***p < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512