Fig. 6From: Extracellular vesicles derived from human umbilical cord mesenchymal stem cells alleviate osteoarthritis of the knee in mice model by interacting with METTL3 to reduce m6A of NLRP3 in macrophageHucMSCs-EVs promote the proliferation and inhibit the apoptosis of chondrocyte by downregulating METTL3 in macrophage. A. Proliferation rate of chondrocytes was determined by flow cytometry analysis after EdU assay. B. Proliferation rate of chondrocyte as determined by immunofluorescence. Scale bar = 200 μm. C. Analysis of flow cytometry results from EdU assay (n = 3). D. Analysis of EdU positive cell rate in each group (n = 3). E. The Annexin V-FITC/PI apoptosis assay for apoptosis rate of chondrocytes determined by flow cytometry analysis. F. Apoptosis rate of chondrocytes as determined by immunofluorescence. Scale bar = 200 μm. G. Analysis of flow cytometry results from the Annexin V-FITC/PI apoptosis assay (n = 3). H. Analysis of TUNEL positive cell rate for each group (n = 3). Data are presented as mean ± SD. ns, no significance; *p < 0.05; **p < 0.01; ***p < 0.001Back to article page