Fig. 3

Ra promoted the differentiation of hiPSCs inti pacemaker cells. a The pacemaker cells rate marked by both CTNT positive and Nkx2.5 negative (cTNT⁺Nkx2.5⁻) in four groups detected by flow cytometry (n = 4). b The pacemaker cells rate marked by both Shox2 positive and Nkx2.5 negative (Shox2⁺Nkx2.5.⁻) in four groups detected by flow cytometry (n = 4). c Cells differentiated from four groups were stained for cTNT (green), Shox2 (red) and DAPI (blue). Magnification, × 200, n = 4