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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Adipose-derived stem cells attenuate acne-related inflammation via suppression of NLRP3 inflammasome

Fig. 2

ADSCs inhibit P. acnes‐induced activation of the NLRP3 inflammasome in monocytes. Cells were pretreated with P. acnes at MOI = 10 for 24 h and co-cultured with ADSCs for 24 or 48 h in a Transwell system. A, E NLRP3, pro-IL-1β, and pro-caspase-1 in cell extracts (CE) and IL-1β and caspase-1 (p10) in supernatants (SN) evaluated by western blot. B, F The secreted IL-1β level in the cell supernatants determined by ELISA (protein level). C RT-PCR for IL-1β (messenger RNA expression). D NLRP3 siRNA (siNLRP3) and caspase-1 siRNA (Sicasp-1) were transfected to knock down NLRP3 expression. Control1 was THP-1 cultured in RPMI-1640 without FBS. Control2 was THP-1 treated with P. acnes for 24 h. SiNC indicates the negative control. n = 5; *p < 0.05; **p < 0.01; ***p < 0.001

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