Fig. 2

Characterization of hUC MSC- EMVs and hUC MSC-exosomes. TEM images of MSC-EMVs and MSC-exosomes. Scale bar: 200 nm (A). Size distribution of MSC-EMVs and MSC-exosomes as measured by NTA (B). The expression levels of TSG101, Alix and CD63 in MSC-EMVs (10 μg) and MSC-exosomes (10 μg) as detected via western blot analysis (C). The yields of EMVs and exosomes measured as the total proteins and particle numbers (n = 3) (D). Exo: exosomes