Fig. 3

The interaction between P300 and c-Myc mediated the increased expression of VEGF in HUVECs induced by WJ-MSCs ECM. a Protein expression of P300 in HUVECs was determined by western blotting. Mean ± S.E.M. n = 3. b Analysis of the interaction between c-Myc and P300 by co-Immunoprecipitation (Co-IP). c–g Matrigel tube formation assay images of cumulative tube length. Bar = 50 μm. Mean ± S.E.M., n = 3. h–l Transwell for HUVECs migration assay and quantitative analysis of migrated HUVEC numbers. Bar = 50 μm. Mean ± S.E.M., n = 3. m–q Immunofluorescently stained for VEGF of HUVECs (magnification: × 400). Bar = 50 μm. Mean ± S.E.M., n = 3. SiRNA: P300 SiRNA. The P value was calculated using one-way ANOVA and independent samples t-test. *P < 0.05, **P < 0.01 versus control