Fig. 5

Integrin αVβ3 mediated the effect of WJ-MSCs ECM on HUVECs. a–e mRNA expressions of integrin α5, αV, β1, β3, and β5 in HUVECs were determined by RT-qPCR. Mean ± S.E.M., n = 5. f, o–s Protein expressions of FAK, p-FAK, P38, p-P38 and VEGF in HUVECs were determined by western blotting. Mean ± S.E.M. n = 3. g–n Matrigel tube formation assay images of cumulative tube length. Bar = 50 μm. HY-100563: integrin αVβ3 specific antagonist. HY-13535A: integrin α5β1 specific antagonist. The P value was calculated using one-way ANOVA and independent samples t-test. *P < 0.05, **P < 0.01 versus control