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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Mesenchymal stem cell-derived exosomes protect against liver fibrosis via delivering miR-148a to target KLF6/STAT3 pathway in macrophages

Fig. 5

MSC-EXO inhibited pro-inflammatory macrophages and promoted anti-inflammatory macrophages in liver. A, B Representative images showed the iNOS + pro-inflammatory macrophages (green) and CD206 + anti-inflammatory macrophages (green) in each group. The nuclei were stained with DAPI (blue), and macrophages were stained with F4/80 (red). Scale bar, 100 μm. C The percentage of iNOS + or CD206 + cells was calculated by Image-Pro Plus. D, E The protein level of iNOS and Arg1 was measured by western blot, and quantification of the relative expression in the bands in different groups was calculated by Image-Pro Plus. F, G The mRNA levels of pro-inflammatory and anti-inflammatory macrophage markers were measured by RT-PCR. H, I Flow cytometry plots showing CD86 (M1 marker) and CD206 (M2 marker) changed in different groups and the percentage of CD86 + or CD206 + cells were analyzed by Flow Jo software

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Stem Cell Research & Therapy

ISSN: 1757-6512