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Fig. 9 | Stem Cell Research & Therapy

Fig. 9

From: Mesenchymal stem cell-derived exosomes protect against liver fibrosis via delivering miR-148a to target KLF6/STAT3 pathway in macrophages

Fig. 9

MiR-148a targetedly suppressed the expression of Kruppel-like factor 6 (KLF6). A The potential binding sites of human miR-148a on KLF6 3′UTR. B Luciferase reporter assay was performed to determine the interaction between miR-148a and KLF6. C–F The protein level of KLF6 and quantification of KLF6 were calculated by Image-Pro Plus. G, H The protein level of KLF6, iNOS and Arg1 was measured by western blot, and quantification of KLF6, iNOS and Arg1 was calculated by Image-Pro Plus. I RT-PCR was applied to measure the mRNA level pro-inflammatory and anti-inflammatory makers in RAW264.7 transfected with KLF6 siRNA. J, K The protein level of iNOS and Arg1 of BMDM was measured by western blot, and quantification of iNOS and Arg1 was calculated by Image-Pro Plus. L RT-PCR was applied to measure the mRNA level pro-inflammatory and anti-inflammatory makers in BMDM transfected with KLF6 siRNA. M, N Western blot was employed to measure the expression of pathway-related proteins. Quantification of p-AKT/AKT and p-STAT3/STAT3 was calculated by Image-Pro Plus

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