Fig. 1
CEFFE improved ovarian function in POI mice. A Schematic design of the animal experiments. B The body weights of mice were measured 2 weeks after treatment with CEFFE (n = 10). C The ovary weights of mice were measured 2 weeks after treatment with CEFFE (n = 10). D Representative images of ovaries from the control, POI, POI + CFL and POI + CFH groups. E Typical morphology of vaginal smears showing different stages of the estrous cycle. a. Proestrus b. Estrus. c. Postestrus. d. Diestrus. Scale bars = 200 μm. F The average length of the estrous cycle (n = 9). G The ratios of regular and irregular estrous cycles of mice in each group (n = 9). H The levels of AMH, E2 and FSH were measured by ELISA at 1, 2, 4 and 8 weeks after treatment with CEFFE (n = 5). I The pathomorphology of ovaries was analyzed by H&E staining. Scale bars = 200 μm. J Quantitative analysis of follicles at different levels in total ovary (n = 4). K The percentage of different follicles in per ovary was analyzed (n = 4). Pri: Primordial follicles, Prim: Primary follicles, Sec: Secondary follicles, Ant: Antral follicles, Health: total number of health follicles (including primordial, primary, secondary and antral follicles), Atr: atretic follicles. All data are represented as the mean ± SD. ns: P > 0.05 represent the compare between two group which was labelled with short line above; *P < 0.05, **P < 0.01, ***P < 0.001, compared with POI group