Fig. 4

CEFFE contributed to the proliferation of CTX-damaged KGN cells. A Cell proliferation was measured by EdU assays. Green fluorescence represents proliferative cells, and blue fluorescence represents the nuclei of all cells. Scale bar = 50 μm. B Semiquantitative results of the EdU assay. C Cell proliferation was measured by CCK-8 assays. D The cell cycle was detected by flow cytometry. E Quantitative analysis of the cell cycle distributions of G0/G1, S, and G2/M phases. CF-L: CEFFE at a concentration of 0.06 µg/µL, CF-M: CEFFE at a concentration of 0.15 µg/µL, CF-H: CEFFE at a concentration of 0.3 µg/µL. The data are presented as the mean ± SD. ns: P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, compared with CTX group