Fig. 7

The mRNA-Seq analysis of mouse ovarian GCs. A Schematic design of the mRNA-Seq analysis of mouse ovarian GCs. POI: POI mice treated with CTX and BUS; POI + CF: POI mice treated with CTX and BUS, followed by high-dose CEFFE (3 µg/µl, 200 µl) treatment. B The heatmap showing DEGs in the control, POI and POI + CF groups with an absolute fold change > 1.5 and P value < 0.05. (n = 3). C Venn diagram showing gene counts in the POI versus control and POI + CF versus POI groups. D Volcano plot showing DEGs in the POI + CF group compared to the POI group. Among the 394 genes with an absolute fold change > 1.5, 219 genes were upregulated, while 175 genes were downregulated. E Top 20 GO terms for DEGs. F Top 20 KEGG terms for DEGs