Fig. 1

The effect of different concentrations of H₂O₂ on HUVECs activity and apoptosis. A The proliferation of HUVECs after treated with graded concentrations (0, 100, 200, and 400 μmol/L) of H₂O₂ at 8, 12, and 24 h measured by CCK8 assay. ns. P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001 vs. 0 μmol/L. B The level of SOD viability of HUVCEs cultured with graded concentrations (0, 100, 200, and 400 μmol/L) of H₂O₂ at 8, 12 and 24 h. ns. P > 0.05, * P < 0.05, ** P < 0.01, **** P < 0.0001 vs. 0 umol/L. C, D The apoptosis of HUVECs after treated with 100 μmol/L H₂O₂ at different time points determined with Annexin V/PI staining by flow cytometry analysis. ns. P > 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. 0 h