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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Oxidative stress-induced endothelial cells-derived exosomes accelerate skin flap survival through Lnc NEAT1-mediated promotion of endothelial progenitor cell function

Fig. 3

Characterization of EPCs and internalization of HUVEC-derived exosomes by EPCs. A Morphology of primary EPCs on the day 5 and 10 of culture. B Fluorescence microscopy evaluation of DiI-Ac-LDL uptake and membrane binding of FITC-UEA-1 by EPCs. DiI-Ac-LDL in red, FITC-UEA-1 in green, DAPI in blue. C Flow cytometry analysis of surface markers of EPCs. Positive expression of CD34, CD309, and CD133, while negative for CD14 and CD45. D Exosomes derived from HUVECs could be taken up by EPCs. Calcein AM (green) and DAPI (blue) were used to stain the cytoplasm and nucleus of EPC, respectively. Exosomes were labeled with DiI (red)

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Stem Cell Research & Therapy

ISSN: 1757-6512