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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Oxidative stress-induced endothelial cells-derived exosomes accelerate skin flap survival through Lnc NEAT1-mediated promotion of endothelial progenitor cell function

Fig. 4

H2O2-HUVEC-Exos enhanced the pro-angiogenic abilities of EPCs in a dose-dependent manner. A Representative images of capillary-like tube formation of EPCs incubated with graded concentration (0–100 μmol/L) of HUVEC-Exos and H2O2-HUVEC-Exos or PBS. EPCs were labeled with Calcein-AM (green). B Quantitative analysis of the number of nodes, the number of branches and the total length of the branches of (A). C Representative images of invaded EPCs stained by DAPI after cultured with graded concentration (0–100 μmol/L) of HUVEC-Exos and H2O2-HUVEC-Exos or PBS. D Quantitative analysis of (C). E. The proliferation of EPCs treated with graded concentration (0–100 μmol/L) of HUVEC-Exos and H2O2-HUVEC-Exos or PBS detected by CCK8. F The VEGF, PDGFC, and PTEN mRNA expression of EPCs after treated with 100 μmol/L HUVEC-Exos and H2O2-HUVEC-Exos. ns. P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. control group

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