Fig. 6
Exosomal Lnc NEAT1 accelerated angiogenesis via the Wnt/β-catenin pathway in EPCs. A The expression of Lnc NEAT1 in HUVECs and H2O2-HUVEC after transfection with si-NEAT1. *** P < 0.001 vs. HUVEC group, ## P < 0.01 vs. H2O2-HUVEC-Exos group. B The expression of Lnc NEAT1 levels in Exos (HUVEC-Exos, si-NEAT1-HUVEC-Exos, H2O2-HUVEC-Exos and si-NEAT1-H2O2-HUVEC-Exos) and in EPCs after cultured with HUVEC-Exos, si-NEAT1-HUVEC-Exos, H2O2-HUVEC-Exos or si-NEAT1-H2O2-HUVEC-Exos. *** P < 0.001 vs. HUVEC-Exos group, ## P < 0.01, #### P < 0.0001 vs. H2O2-HUVEC-Exos group. C Representative images of invaded EPCs stained by DAPI after cultured with HUVEC-Exos, si-NEAT1-HUVEC-Exos, si-NEAT1-HUVEC-Exos + HLY78, H2O2-HUVEC-Exos, si-NEAT1- H2O2-HUVEC-Exos or si-NEAT1-H2O2-HUVEC-Exos + HLY78. D Quantitative analysis of (C). E Representative images of capillary-like tube formation generated by EPCs cultivated with HUVEC-Exos, si-NEAT1-HUVEC-Exos, si-NEAT1-HUVEC-Exos + HLY78, H2O2-HUVEC-Exos, si-NEAT1-H2O2-HUVEC-Exos, si-NEAT1-H2O2-HUVEC-Exos + HLY78 for 8 h. EPCs were labeled with Calcein-AM (green). F Quantitative analysis of (E). G The β-catenin, c-myc and cyclin D1 protein levels in EPCs after treated with HUVEC-Exos, si-NEAT1-HUVEC-Exos, si-NEAT1-HUVEC-Exos + HLY78, H2O2-HUVEC-Exos, si-NEAT1-H2O2-HUVEC-Exos, si-NEAT1-H2O2-HUVEC-Exos + HLY78C-Exos. ns. P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001 versus HUVEC-Exos group, ns. P > 0.05, # P < 0.05, ## P < 0.01, ### P < 0.001 vs. H2O2-HUVEC-Exos group