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Fig. 10 | Stem Cell Research & Therapy

Fig. 10

From: Implantation of dedifferentiated fat cells ameliorated antineutrophil cytoplasmic antibody glomerulonephritis by immunosuppression and increases in tumor necrosis factor-stimulated gene-6

Fig. 10

Effects of implantation of DFAT cells on the population of regulatory T cells in SCG mice. Spleen was removed and homogenized 4 weeks after implantation of 106 DFAT cells in SCG mice. Cells from spleen were labeled with fluorogenic antibodies to evaluate the proportion of CD4+ CD25+ FOXP3+ regulatory T cells. Cells were fixed and permeabilized with a FOXP3 Staining Buffer Set according to the manufacturer’s instructions and included a blocking step with 2% rat serum. Flow cytometry was performed with a FACSAria system, and data were analyzed using FlowJo 7.6.5 software. DFAT dedifferentiated fat

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Stem Cell Research & Therapy

ISSN: 1757-6512