Fig. 2

Overexpression mmu_circ_0001052 in ADSCs-derived exosomes promoted proliferation, migration and angiogenesis of high glucose-induced HUVECs. A the expression of miR-106a-5p and mmu_circ_0001052 in high glucose-induced HUVECs. B the transfection efficiency via fluorescence microscope. C the expression of mmu_circ_0001052 in ADSCs transfected with different vectors (exosomes in exo group was extracted from normal ADSCs, these in exo+oe-vector group from ADSCs transfected with empty vector, and these in exo+oe-mmu_circ_0001052 group from ADSCs transfected with oe-mmu_circ_0001052). D the fusion of exosomes into cells via immunofluorescence. E apoptosis by flow cytometry. F proliferation by MTT. G migration by transwell. H angiogenesis via tube formation assay. All data were obtained from at least three replicate experiments. *P < 0.05, **P < 0.01. N = 3. One-way ANOVA was used to analyze the differences among groups. Afterward, post-pairwise comparison was conducted by Dunnett’s multiple comparison test. Comparison among multiple groups at different time points was analyzed by repeated measures ANOVA