Fig. 3

The effects of AG on antioxidative stress in BMSCs under GSDH. In (H), the samples derive from the same experiment and that the blots were processed in parallel and β-actin was used as the control. A DCFH-DA staining was used to measure ROS; stained cells were observed by fluorescence microscopy, and B statistical analysis of the MFI of ROS in each group (n = 20 cells). C, D Flow cytometry was used to measure the MFI of ROS in each group (n = 3). E–G qRT-PCR analysis of the effect of AG on antioxidation-related genes (GCLC, GSH-px, and CAT) in BMSCs (n = 3). H Western blot analysis of the protein levels of GCLC, GPX4, CAT and SOD1 (n = 3). Statistical analysis of data from each group is shown in (I-L), normalized with β-actin (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001