Fig. 4

The effects of AG on GSDH-induced apoptosis and changes in ∆Ψm. A Flow cytometry with Annexin V-FITC and PI staining, and cells located in quadrants Q2 and Q3 were considered apoptotic cells (n = 3). Quantitative analysis of the apoptotic rate is shown at right. B The ∆Ψm was determined by JC-1 staining, Blue: Hoechst 33,342, Red: J–aggregates, Green: monomers. Statistical analysis of the red/green MFI ratio is shown at right (n = 20 cells). Scale bars: 50 μm. **P < 0. 01, ***P < 0. 001, ****P < 0.0001