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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Andrographolide protects bone marrow mesenchymal stem cells against glucose and serum deprivation under hypoxia via the NRF2 signaling pathway

Fig. 5

The effects of AG on the mitochondrial morphology and number. A The mitochondrial morphology was observed by a laser confocal microscope, Blue: Hoechst 33,342, Green: MitoTracker, scale bars: 25 μm. B Analysis of the MFI of each group by using ImageJ software (n = 20 cells). C The number of mitochondria was assessed by flow cytometry (n = 3). D Analysis of the MFI of mitochondria in each group. E qRT-PCR analysis of the mitochondrial DNA copy number. F Fluo-4 AM staining was used to assess Ca2+ by laser confocal microscopy. G The MFI of each group was analyzed by ImageJ software (n = 20 cells). H Cellular Ca2+ was determined by flow cytometry (n = 3). I Analysis of the MFI of fluo-4AM in each group. Scale bars: 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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