Fig. 8

AG regulates apoptosis and oxidative stress in BMSCs under GSDH through NRF2 pathway. In A the samples derive from the same experiment and that the blots were processed in parallel, β-actin was used as a loading control. A Western blot analysis of the expression of NRF2 and HO-1, with β-actin as a control (n = 3), and B statistical analysis in each group, normalized with β-actin. C Flow cytometry with Annexin V-PE and 7-AAD staining (n = 3). Statistical analysis is shown on the right. D The number of DCFH-DA-positive cells was assessed by using a flow cytometer (n = 3), and statistical analysis of the MFI in each group is shown on the right. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001