Fig. 2

H-exos mediate the differentiation of MSCs into MT-CAFs by activating TRPC3. A, B The expression of TRPC3 was detected using time series analysis at days 0, 1, 3, 7, and 10, as well as WB at days 0, 3, 5, 7, and 9. C, D Time-series analysis and WB evaluation of CREB1 expression at days 0, 1, 3, 7, and 10. E The cellular Ca²⁺ levels were examined by Furo-4 staining at day 5, the fluorescence intensity was obviously enhanced after adding H-exos (Green). F The expression of TRPC3 and a-SMA was detected by immunofluorescence double staining (TRPC3: red; a-SMA: green)