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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: MiR-152-5p suppresses osteogenic differentiation of mandible mesenchymal stem cells by regulating ATG14-mediated autophagy

Fig. 1

Inhibition of autophagy and upregulation of miR-152-5p in OVX MMSCs-M. a Alizarin Red S staining was performed to illustrate mineralized nodule after 14 days of osteogenic induction, scar bar = 200um. b. ALP staining and ALP activity assays were performed on 7 days of osteogenic induction, scar bar = 200um. c QRT-PCR and western blots assay were performed to detect the osteogenesis-related markers including Runx2, Alp and Ocn (n = 3) after 14 days of osteogenic induction at RNA and protein level. d The expression of LC3B and p62 protein (n = 3) in OVX and sham MMSCs-M were detected by western blots assays after 14 days of osteogenic induction. e Immunofluorescence detected the protein LC3B (green) in sham and OVX MMSCs-M with blue (DAPI staining) nucleus. Scar bar = 50um. f The number of autophagic vacuoles was observed by TEM, scar bar = 2um. g The expression of ten miRNAs in OVX and sham MMSCs-M. Data were shown as mean ± SD (p < 0.05*, p < 0.01**, p < 0.0005***, p < 0.0001****)

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