Fig. 3

miR-152-5p inhibits autophagy and osteogenic differentiation in MMSCs-M. a MiR-152-5p inhibitor and inhibitor NC were transfected into OVX MMSCs-M. Western blots assays detected the level of LC3B and p62 in control, inhibitor, inhibitor NC groups (n = 3). b Immunofluorescence staining of LC3B. c Alizarin Red S staining was performed to illustrate mineralized nodule in control, inhibitor, inhibitor NC groups. d ALP staining and activity of the above groups. e The expression of ALP, RUNX2, OCN were detected by western blots assay of these three groups (n = 3). Data were shown as mean ± SD (p < 0.05*, p < 0.01**, p < 0.0005***, p < 0.0001****)