Fig. 4

Downregulation of miR-152-5p regulates osteogenic differentiation via promoting autophagy in OVX MMSCs-M. a RUNX2, ALP, OCN were detected by Western blots in groups of NC, inhibitor, inhibitor + 3-MA, inhibitor + siATG5 (n = 3). b QRT-PCR was performed to assess the expression of Runx2, Alp, Ocn in above groups (n = 3). c, d. Alizarin Red S staining and ALP staining and activity of these groups. Data were shown as mean ± SD (p < 0.05*, p < 0.01**, p < 0.0005***, p < 0.0001****)