Fig. 6
miR-152-5p/ATG14-mediated autophagy regulated osteogenic differentiation by reducing the elevated ROS. a ROS detection of sham and OVX’ MMSCs-M. Scar bar = 50um. b H2O2 (100um) was applied to OVX MMSCs-M in order to increase the ROS level. ROS detection of different groups in inhibitor NC + siNC, inhibitor NC + siNC + H2O2 (100uM), miR-152-5p inhibitor + siNC, miR-152-5p inhibitor + siNC + H2O2 (100uM), miR-152-5p inhibitor + siATG14 + H2O2. c Western blotting assays were performed to verify that ATG14-mediated autophagy regulated osteogenic differentiation by reducing the elevated ROS (n = 3). Scar bar = 50um. Data were shown as mean SD (p < 0.05*, p < 0.01**, p < 0.0005***, p < 0.0001****)