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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Meiosis resumption in human primordial germ cells from induced pluripotent stem cells by in vitro activation and reconstruction of ovarian nests

Fig. 3

Generation of SYCP3-mkate2 reporter knock-in human iPSC lines. A Schematic illustration of the SYCP3 locus and the donor construct carrying T2A-mKate2 and hEF1a-Neo-pA fragments. Black boxes indicate the exons. B PCR screening of homologous recombinants for SYCP3-mkate2 and the removal of the selection cassettes (loxP-hEF1a-Neo-pA-loxP). Clones bearing SYCP3-mkate2 were selected for use in the subsequent studies. C Normal karyotype of SYCP3-mkate2 reporter knock-in human iPSC lines

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Stem Cell Research & Therapy

ISSN: 1757-6512