Fig. 4

In vitro activation of dormant human PGCs/oogonia overcomes meiosis phase arrest through the Wnt signaling pathway. A Percentage of SYCP3-mkate2-positive cells among human PGCs/oogonia treated with the GSK3 inhibitor was 8.2 ± 1.32%, while few cells were mkate2-positive. B Activated PGCs aggregated with human fetal gonad somatic cells differentiated into SCP3-positive meiocytes, in contrast to the pseudoaggregates formed from only human gonad somatic cells that showed no SCP3 meiocytes and were used as a negative control. B-1: Negative control; B-2: magnifications of the negative control; B-3: positive control; and B-4: magnifications of the negative control. C Expression of DDX4 in the ovary reconstituted with a mixture of human activated PGCs and fetal somatic cells. D Increased relative expression levels of SP3 and Dmc1 in PGCs induced from HEF-iPSCs. D Gene expression of GSK-3β, β-catenin, CDK1, Cyclin B1, SYCP3 and REC8 in the activated group and nonactivated group. E Proposed effect of the Wnt signaling pathway on meiotic resumption and progression in the differentiation of PGCs/oogonia