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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Human fibroblasts facilitate the generation of iPSCs-derived mammary-like organoids

Fig. 4

Establishing MLOs from human iPSCs is improved by HFF. A Representative bright-field images of MLOs generated from human iPSCs with (lower panel) or without HFF (upper panel) at day 30. Bar, 50 μm. B Branching ratios of MLOs and MLOs-HFF. C Representative immunofluorescence images of MLOs and MLOs-HFF at day 30. Bar, 100 μm. D Sizes of MLOs and MLOs-HFF at different days. Day 10: MLOs (n = 136), MLOs-HFF (n = 107); day 17: MLOs (n = 131), MLOs-HFF (n = 121); day 25: MLOs (n = 83), MLOs-HFF (n = 84); day 30: MLOs (n = 66), MLOs-HFF (n = 78). E Percentage of large organoids (diameter > 200 μm) of MLOs and MLOs-HFF at day 30. F Representative hematoxylin and eosin staining of MLOs and MLOs-HFF at day 30 (left); percentage of organoids displaying duct-like structure in MLOs (n = 13) and MLOs-HFF (n = 39) at day 30 (right). G Representative immunohistochemical staining of MLOs and MLOs-HFF at day 30. Two-tailed Student t test, *p < 0.05, **p < 0.01, ***p < 0.001

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