Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Cyst stem cell lineage eIF5 non-autonomously prevents testicular germ cell tumor formation via eIF1A/eIF2γ-mediated pre-initiation complex

Fig. 1

Reduction in eIF5 in CySCs blocked cyst cell differentiation and caused GSC-like mass accumulation. A Relative mRNA level of eIF5 in control and Tj > eIF5 RNAi testes. B Immunostaining of Zfh1 (red) and Eya (green) at the apex of control, Tj > eIF5 RNAi, and Tj > eIF5 OE; eIF5 RNAi testes. C The number of Eya-positive cells at the apex of testes. Control, n = 17; Tj > eIF5 RNAi, n = 20; Tj > eIF5 OE; eIF5 RNAi, n = 11. D The number of Zfh1-positive cells at the apex of testes. Control, n = 17; Tj > eIF5 RNAi, n = 20; Tj > eIF5 OE; eIF5 RNAi, n = 11. E The number of pointed fusomes at the apex of testes. Control, n = 12; Tj > eIF5 RNAi, n = 11; Tj > eIF5 OE; eIF5 RNAi, n = 5. F Immunostaining of Vasa (red) and 1B1 (green) at the apex of control, Tj > eIF5 RNAi, and Tj > eIF5 OE; eIF5 RNAi testes. DNA was stained with Hoechst (blue). (**P < 0.01; ***P < 0.001, n.s. indicates non-significant. Scale bar: 50 μm)

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512