Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Cyst stem cell lineage eIF5 non-autonomously prevents testicular germ cell tumor formation via eIF1A/eIF2γ-mediated pre-initiation complex

Fig. 3

eIF5 deficiency in CySCs mediated hyperproliferation and affected spermatogenesis. A Immunostaining of PH3 (green) and FasIII (red) at the apex of control, Tj > eIF5 RNAi, and Tj > eIF5 OE; eIF5 RNAi testes. B The number of PH3-positive cells at the apex of testes. Control, n = 12; Tj > eIF5 RNAi, n = 9; Tj > eIF5 OE; eIF5 RNAi, n = 9. C The number of EdU-positive cells at the apex of testes. Control, n = 11; Tj > eIF5 RNAi, n = 11; Tj > eIF5 OE; eIF5 RNAi, n = 8. D EdU staining (green) at the apex of control, Tj > eIF5 RNAi, and Tj > eIF5 OE; eIF5 RNAi testes. E F-actin staining (red) of control, Tj > eIF5 RNAi, and Tj > eIF5 OE; eIF5 RNAi testicular tails. F The number of individualization complexes (ICs) in testicular tails. Control, n = 12; Tj > eIF5 RNAi, n = 11; Tj > eIF5 OE; eIF5 RNAi, n = 12. G The number of elongated spermatid clusters in testicular tails. Control, n = 12; Tj > eIF5 RNAi, n = 11; Tj > eIF5 OE; eIF5 RNAi, n = 12. DNA was stained with Hoechst (blue). (***P < 0.001. n.s. indicates nonsignificant. Scale bars: 50 µm)

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512