Skip to main content
Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Cyst stem cell lineage eIF5 non-autonomously prevents testicular germ cell tumor formation via eIF1A/eIF2γ-mediated pre-initiation complex

Fig. 7

CySC lineage eIF5 regulates eIF1A and eIF2γ in Drosophila testes. A Knockdown efficiency of eIF1A and eIF2γ in control and corresponding RNAi testes, respectively. n = 3 per group. B Immunostaining of Zfh1 (red) and Eya (green) at the apex of control, Tj > eIF1A RNAi, and Tj > eIF2γ RNAi testes. C Immunostaining of Vasa (red) and 1B1(green) at the apex of control, Tj > eIF1A RNAi, and Tj > eIF2γ RNAi testes. D Relative mRNA level of eIF1A and eIF2γ in control and Tj > eIF5 RNAi testes. n = 3 per group. E Relative mRNA level of eIF5 in control, Tj > eIF1A RNAi, and Tj > eIF2γ RNAi testes. n = 3 per group. F Proportion of testes lacking morphological structures in Tj > eIF1A RNAi and Tj > eIF5 OE; eIF1A RNAi testes. Tj > eIF1A RNAi, n = 136; Tj > eIF5 OE; eIF1A RNAi, n = 61. G Proportion of testes lacking morphological structures in Tj > eIF2γ RNAi and Tj > eIF5 OE; eIF2γ RNAi testes. Tj > eIF2γ RNAi, n = 124; Tj > eIF5 OE; eIF2γ, RNAi n = 53. H Immunostaining of Zfh1 (red) and Eya (green) at the apex of Tj > eIF5 OE; eIF1A RNAi and Tj > eIF5 OE; eIF2γ RNAi testes. I Immunostaining of Vasa (red) and 1B1(green) at the apex of Tj > eIF5 OE; eIF1A RNAi and Tj > eIF5 OE; eIF2γ RNAi testes. DNA was stained with Hoechst (blue). (***P < 0.001. Scale bars: 50 µm)

Back to article page