Fig. 2

Late passage (LP) MSC(AT) have a senescent transcriptome signature. A Gene set enrichment analysis demonstrates increased expression of genes known to be upregulated in senescence (shown in red) and reduced expression of genes downregulated in senescence (shown in blue) in LP-MSC(AT) and shows DPP4 among core enrichment genes; n = 1 pediatric and n = 1 adult, where EP = p6 and LP = p30. B–G Relative expression of selected transcripts from RNAseq analysis tested by quantitative RT-PCR over HPRT housekeeping gene in 9 additional samples. Expression of B CDKN2A (p16), C CDKN1A (p21), D DPP4 (CD26), E HES1, F SPP1 and G COL4A1 over the house-keeping gene HPRT. Data presented as mean ± SD, comparisons done with paired Wilcoxon test, *p < 0.05, **p < 0.01