Fig. 4

CD26^high MSC(AT) are less immunosuppressive than CD26^low MSC(AT). A Flow cytometry gating strategy for FACS separation of MSC(AT) based on CD26 surface abundance: CD26^low and CD26^high populations. B Immunopotency assay (i.e., MSC inhibition of proliferating CD4⁺ T cells). CD3CD28 activated T-cells were co-cultured with either CD26^low or CD26^high MSC(AT) at a 1:16 MSC:PBMC ratio. n = 4 adult and 1 pediatric MSC(AT). Data represent mean ± SD, comparisons done with paired Wilcoxon tests, *p < 0.05