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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: C/EBPα regulates the fate of bone marrow mesenchymal stem cells and steroid-induced avascular necrosis of the femoral head by targeting the PPARγ signalling pathway

Fig. 2

C/EBPα inhibits osteogenic differentiation of BMSCs. a The specific surface markers of BMSCs analysed by flow cytometry. b–d The establishment of knockdown or overexpression of C/EBPα determined by RT–qPCR and western blot analysis, respectively. e, f Alizarin red staining of osteogenic differentiation of BMSCs in each group and quantitative analysis by ImageJ. g–i The protein or mRNA levels of osteogenesis-related genes in BMSCs. ***P < 0.001, **P < 0.01, *P < 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512