Fig. 2

Effects of different H₂O₂ concentrations on NPMSCs proliferation. a The effect of H₂O₂ on NPMSC proliferation detected by the CCK-8 assay. (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test). b and c Detection of proliferated NPMSCs (red) under different H₂O₂ concentrations by the EdU-555 proliferation assay kit and quantification. Nuclei were counterstained with Hoechst 33358 (blue) (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test). Scale bar = 200 μm. d and e Western blotting analysis of cyclin D1 and P16 expression in NPMSCs exposed to different concentrations of H₂O₂ for 12 h (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test). f and g Western blotting analysis of p-Lats1, p-Mst1, p-YAP, and YAP expressions in NPMSCs exposed to different concentrations of H₂O₂ for 12 h (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test)