Fig. 3

Effect of different H₂O₂ concentrations on ROS and MMP in NPMSCs. a and b The intracellular ROS in NPMSCs that received different H₂O₂ concentrations were detected by flow cytometry and quantified (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test). c and d The intracellular ROS in NPMSCs that received 75 μM H₂O₂ pretreatment were detected by flow cytometry and quantified (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test). e and f The MMP of NPMSCs received 75 μM H₂O₂ pretreatment detected by CMXRos and quantified (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test, ^##P < 0.01 compared to the 300 μM H₂O₂ group). Scale bar = 50 μm