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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Pretreatment of nucleus pulposus mesenchymal stem cells with appropriate concentration of H2O2 enhances their ability to treat intervertebral disc degeneration

Fig. 4

Effect of different H2O2 concentrations on the Keap1-Nrf2 signalling pathway in NPMSCs. a and b Western blotting analysis of Keap1 and Nrf2 expression in NPMSCs exposed to different concentrations of H2O2 (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test. #P < 0.05 compared to the 300 μM H2O2 group). c and d Western blotting analysis of Brd4 expression in NPMSCs exposed to different concentrations of H2O2 (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test. #P < 0.01 compared to the 300 μM H2O2 group). e and f Western blotting analysis estimating the effect of 75 μM H2O2 pretreatment on Keap1, Nrf2, and Brd4 expression and quantification when NPMSCs were subsequently exposed to 300 μM H2O2 (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test. ##P < 0.01 compared to the 300 μM H2O2 group)

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