Fig. 4

FbMSC application promotes the recovery of axonal injury in TBI mice. MAP2 immunofluorescence and fluorescence intensity quantification in peri-impact area and hippocampus in each group at 4 d (a, b) and 28 d (c, d) post-injury. Scale bar, 20 μm. e Representative 3D reconstruction images of MAP2 staining in hippocampus in each group at 4 d post-injury. Scale bar, 150 μm. Doublecortin (DCX) immunofluorescence image (f) and fluorescence intensity quantification (g) in each group at 14 d post-injury. Scale bar, 20 μm. h Expression of BDNF was detected by qRT-PCR at 14 d post-injury. (n = 3–4 mice per group; Data are presented as the mean ± standard error; *, **, ***, and **** indicate significance at p < 0.05, p < 0.01, p < 0.001, and p < 0.0001, respectively.)