Fig. 7

FGF1 from FbMSCs promotes glutamate uptake of C8-D1A cells and alleviates neuron excitotoxicity. a Representative images of MAP2 stained neurons treated with indicated doses of glutamate. Scale bar, 20 μm. Branch point (b) and Dendritic length (c) were quantified according to MAP2 staining. Representative staining images (d) and quantification of cell viability (e) according to calcein-AM (green)/ethidium homodimer (red) staining on HT22 cells. Scale bar, 100 μm. f Glutamate uptake of C8-D1A cells stimulated with indicated doses of FGF1. g Glutamate uptake of C8-D1A cells stimulated with indicated dose of TNFα (150 ng/mL), IL1β (50 ng/mL) and FGF1 (50 ng/mL). Representative images (h) and quantification of neuron branches (i) of MAP2-stained neurons in indicated groups. Scale bar, 20 μm. Representative images (j) and quantification of cell viability (k) of calcein-AM (green)/ethidium homodimer (red) staining of HT22 cells in the indicated groups. Scale bar, 100 μm. l Expression level of FGF1 mRNA in FbMSCs after transfected with siFGF1 and siNC. m Western blot analysis demonstrated levels of FGF1 after transfected with siFGF1 and siNC. n Western blot analysis demonstrated levels of BDNF in lesion areas of mice at 4 days. o mRNA level of BDNF and NGF was detected by qRT-PCR. p Representative images of active caspase-3 staining in the lesion areas from each group at 4 days. Scale bar, 20 μm. (n = 3–4 mice per group; Data are presented as the mean ± standard error; *, **, ***, and **** indicate significance at p < 0.05, p < 0.01, p < 0.001, and p < 0.0001, respectively.)