Fig. 6

PSC-derived SG placodes differentiated into mature salivary ducts in vivo. A Schematic of transplantation procedures and photographs of mouse kidneys with grafted SG placodes after 30 days. (Black arrows marked grafted tissues). B Hematoxylin and eosin staining showed distinct outgrowths after transplantation for 30 days. Scale bars: 500 μm. C, D The excretory ducts (ED) and striated ducts (SD) were formed after transplantation of SG placodes under mouse kidney subcapsular. The mature ducts also expressed the high level of Krt19 (C) and Sox9 (D). Scale bars: 200 μm (C, D) and 100 μm (C’ and D’, magnified images, marked by yellow dotted boxes)